Wyniki 5 etapu z dnia 15.03.2009 Loreto – Macerata, dystans 30 km (jazda na czas)
| 1 Andreas Klöden (Ger|Astana) | 0:41:32 | |
| 2 Stijn Devolder (Bel|Quick Step) | 0:00:20 | |
| 3 Thomas Lövkvist (Swe|Columbia-Highroad) | 0:00:21 | |
| 4 Michele Scarponi (Ita|Serramenti PVC Diquigiovanni-Androni Giocattoli) | ||
| 5 Mikhail Ignatiev (Rus|Katusha) | 0:00:32 | |
| 6 Robert Gesink (Ned|Rabobank) | 0:00:40 | |
| 7 Stefano Garzelli (Ita|Acqua & Sapone-Caffè Mokambo) | 0:00:41 | |
| 8 Edvald Boasson Hagen (Nor|Columbia-Highroad) | 0:00:52 | |
| 9 Linus Gerdemann (Ger|Milram) | 0:00:58 | |
| 10 Jérôme Coppel (Fra|Française des Jeux) | 0:01:00 | |
| Klasyfikacja generalna po 5 etapach |
||
| 1 Andreas Klöden (Ger|Astana) | 16:51:13 | |
| 2 Thomas Lövkvist (Swe|Columbia-Highroad) | 0:00:06 | |
| 3 Michele Scarponi (Ita|Serramenti PVC Diquigiovanni-Androni Giocattoli) | 0:00:21 | |
| 4 Stefano Garzelli (Ita|Acqua & Sapone-Caffè Mokambo) | 0:00:41 | |
| 5 Davide Rebellin (Ita|Serramenti PVC Diquigiovanni-Androni Giocattoli) | 0:01:02 | |
| 6 Vincenzo Nibali (Ita|Liquigas) | 0:01:07 | |
| 7 Linus Gerdemann (Ger|Milram) | 0:01:22 | |
| 8 Ivan Basso (Ita|Liquigas) | 0:01:25 | |
| 9 Ryder Hesjedal (Can|Garmin-Slipstream) | 0:01:26 | |
| 10 Johan Van Summeren (Bel|Silence-Lotto) | 0:01:34 |
